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anti p ikk 2697t  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti p ikk 2697t
    Anti P Ikk 2697t, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1242 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+ikk%CE%B2/Phospho-IKKalpha%2Fbeta+(Ser176%2F180)+Rabbit+mAb/pm41866004-71-1-9
    Average 96 stars, based on 1242 article reviews
    anti p ikk 2697t - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Western Blot:

    Article Title: Macrophage WEE1 Directly Binds to and Phosphorylates NF-κB p65 Subunit to Induce Inflammatory Response and Drive Atherosclerosis.
    Article Snippet: .. Antibodies against GAPDH (#5174, 1:1000), p-IKKβ (#2697, 1:1000), IKKβ (#8943, 1:1000), NF-κB p65 (Transcription factor p65, #8242, 1:1000 for western blotting and 1:200 for im- munofluorescence staining), p-p65 (#3033, 1:1000 for western blotting and 1:200 for immunofluorescence staining), IκBα (#9242, 1:1000), WEE1 (#13 084, 1:1000) and p-WEE1 (S642, #4910, 1:1000 for western blotting and 1:200 for immunofluorescence staining) were purchased from Cell Signaling Technology (Danvers, MA, USA). .. Antibodies against F4/80 (#ab6640, 1:200), α-SMA (#ab7817, 1:200), iNOS (#ab178945, 1:200), and Lamin B1 (#ab133741, 1:1000) were purchased from Abcam (Cambridge, UK).

    Article Title: A novel Lactobacillus delbrueckii-based topical spray promotes wound healing and inhibits dermal fibrosis: A probiotic biomaterial for integrated scar management
    Article Snippet: Impaired wound healing and hypertrophic scarring (HS) represent major clinical challenges, often resulting in excessive collagen deposition, persistent inflammation, and disorganized tissue remodeling.. These pathological processes not only delay wound closure but also lead to unsightly and functionally restrictive scars.. Despite various therapeutic attempts, effective strategies to simultaneously promote epithelial regeneration and prevent fibrotic scar formation remain limited.

    Staining:

    Article Title: Macrophage WEE1 Directly Binds to and Phosphorylates NF-κB p65 Subunit to Induce Inflammatory Response and Drive Atherosclerosis.
    Article Snippet: .. Antibodies against GAPDH (#5174, 1:1000), p-IKKβ (#2697, 1:1000), IKKβ (#8943, 1:1000), NF-κB p65 (Transcription factor p65, #8242, 1:1000 for western blotting and 1:200 for im- munofluorescence staining), p-p65 (#3033, 1:1000 for western blotting and 1:200 for immunofluorescence staining), IκBα (#9242, 1:1000), WEE1 (#13 084, 1:1000) and p-WEE1 (S642, #4910, 1:1000 for western blotting and 1:200 for immunofluorescence staining) were purchased from Cell Signaling Technology (Danvers, MA, USA). .. Antibodies against F4/80 (#ab6640, 1:200), α-SMA (#ab7817, 1:200), iNOS (#ab178945, 1:200), and Lamin B1 (#ab133741, 1:1000) were purchased from Abcam (Cambridge, UK).

    Immunofluorescence:

    Article Title: Macrophage WEE1 Directly Binds to and Phosphorylates NF-κB p65 Subunit to Induce Inflammatory Response and Drive Atherosclerosis.
    Article Snippet: .. Antibodies against GAPDH (#5174, 1:1000), p-IKKβ (#2697, 1:1000), IKKβ (#8943, 1:1000), NF-κB p65 (Transcription factor p65, #8242, 1:1000 for western blotting and 1:200 for im- munofluorescence staining), p-p65 (#3033, 1:1000 for western blotting and 1:200 for immunofluorescence staining), IκBα (#9242, 1:1000), WEE1 (#13 084, 1:1000) and p-WEE1 (S642, #4910, 1:1000 for western blotting and 1:200 for immunofluorescence staining) were purchased from Cell Signaling Technology (Danvers, MA, USA). .. Antibodies against F4/80 (#ab6640, 1:200), α-SMA (#ab7817, 1:200), iNOS (#ab178945, 1:200), and Lamin B1 (#ab133741, 1:1000) were purchased from Abcam (Cambridge, UK).

    Incubation:

    Article Title: Denosumab Regulates Titanium Particles-Induced Macrophages Inflammation and Osteolysis by Targeting the ST18/PARP1 Interaction.
    Article Snippet: Protein samples were denatured in loading buffer (LT101S, Epizyme Biotech, China) at 95°C for 10 min, separated by 10% SDS- PAGE (Shanghai Epizyme Biomedical Technology Co. Ltd., China), and transferred onto PVDF membranes (3010040001, Sigma- Aldrich, USA). .. The membranes were blocked with 5% BSA (CW0043S, CW Biotech, China) for 1 h at room temperature and then incubated overnight at 4°C with the following primary antibodies: p- IKKβ (rabbit, 2697, CST, USA), IKKβ (rabbit, 8943, CST, USA), p- p65 (rabbit, 3033, CST, USA), p65 (rabbit, 8242, CST, USA), p- IκBα (rabbit, 2859, CST, USA), IκBα (mouse, 4814, CST, USA), GAPDH (rabbit, 5174, CST, USA), ST18 (rabbit, PA5- 116320, Invitrogen, USA), TNF- α (rabbit, A11534, ABclonal, China), IL- 6 (rabbit, 12912, CST, USA), and IL- 1β (rabbit, 31202, CST, USA). .. After washing three times with TBST, membranes were incubated with HRP- conjugated goat anti- rabbit secondary antibody (7074, CST, USA) for 1 h at room temperature.

    Article Title: Nuciferine Attenuates Cancer Cachexia-Induced Muscle Wasting in Mice via HSP90AA1.
    Article Snippet: The SDS- PAGE gel was used to separate the protein samples, which were then transferred to a polyvinylidene fluoride membrane (Roche, Switzerland). .. Following the blocking process, the membranes were subjected to overnight incubation at 4°C with homologous primary antibodies aimed at GAPDH (1:40 000, Proteintech, 60004- 1- Ig, Wuhan, China), AKT(1:1000, Cell Signaling Technology, 92772S, MA, United States), p- AKT (Ser473,1:1000, ABclonal, AP0140,Wuhan, China), p- mTOR (Ser2448,1:1000, Cell Signaling Technology, 5536T, MA, United States), mTOR (1:1000, Cell Signaling Technology, 2983T, MA, United States), HSP90AA1 (1:250, ABclonal, A23880, Wuhan, China), TNF- α (1:1000, ABclonal, A0277, Wuhan, China), p- IKKβ (Ser177/181, 1:1000, Cell Signaling Technology, 2694T, MA, United States), IKKβ (1:1000, Cell Signaling Technology, 2678T, MA, United States), IL- 6 (1:1000, ABclonal, A0286, Wuhan, China), NFκB (1:5000, Abmart, T55034S, Shanghai, China), p- NF- κB (Ser536,1:1000, Cell Signaling Technology, 3033T, MA, United States), MyHC (1 μg/mL, R&D Systems, CAEI0822101, MN, United States), Atrogin1 (1:1000, Abways, CY8766, Shanghai, China) and MuRF1 (1:1000, Proteintech, 55 456- 1- AP, Wuhan, China), and then, an ECL plus kit was used to produce the blots (Proteintech, Wuhan, China). .. A chemiluminescence detection system (Syngene, Gene Gnome XRQ, Cambridge, Cambridgeshire, United Kingdom) was utilized to visualize the protein bands, and ImageJ software (NIH, MD, United States) was utilized for analysis.

    Article Title: Denosumab Regulates Titanium Particles‐Induced Macrophages Inflammation and Osteolysis by Targeting the ST18 / PARP1 Interaction
    Article Snippet: Protein samples were denatured in loading buffer (LT101S, Epizyme Biotech, China) at 95°C for 10 min, separated by 10% SDS‐PAGE (Shanghai Epizyme Biomedical Technology Co. Ltd., China), and transferred onto PVDF membranes (3010040001, Sigma‐Aldrich, USA). .. The membranes were blocked with 5% BSA (CW0043S, CW Biotech, China) for 1 h at room temperature and then incubated overnight at 4°C with the following primary antibodies: p‐IKKβ (rabbit, 2697, CST, USA), IKKβ (rabbit, 8943, CST, USA), p‐p65 (rabbit, 3033, CST, USA), p65 (rabbit, 8242, CST, USA), p‐IκBα (rabbit, 2859, CST, USA), IκBα (mouse, 4814, CST, USA), GAPDH (rabbit, 5174, CST, USA), ST18 (rabbit, PA5‐116320, Invitrogen, USA), TNF‐α (rabbit, A11534, ABclonal, China), IL‐6 (rabbit, 12912, CST, USA), and IL‐1β (rabbit, 31202, CST, USA). .. After washing three times with TBST, membranes were incubated with HRP‐conjugated goat anti‐rabbit secondary antibody (7074, CST, USA) for 1 h at room temperature.

    Blocking Assay:

    Article Title: Nuciferine Attenuates Cancer Cachexia-Induced Muscle Wasting in Mice via HSP90AA1.
    Article Snippet: The SDS- PAGE gel was used to separate the protein samples, which were then transferred to a polyvinylidene fluoride membrane (Roche, Switzerland). .. Following the blocking process, the membranes were subjected to overnight incubation at 4°C with homologous primary antibodies aimed at GAPDH (1:40 000, Proteintech, 60004- 1- Ig, Wuhan, China), AKT(1:1000, Cell Signaling Technology, 92772S, MA, United States), p- AKT (Ser473,1:1000, ABclonal, AP0140,Wuhan, China), p- mTOR (Ser2448,1:1000, Cell Signaling Technology, 5536T, MA, United States), mTOR (1:1000, Cell Signaling Technology, 2983T, MA, United States), HSP90AA1 (1:250, ABclonal, A23880, Wuhan, China), TNF- α (1:1000, ABclonal, A0277, Wuhan, China), p- IKKβ (Ser177/181, 1:1000, Cell Signaling Technology, 2694T, MA, United States), IKKβ (1:1000, Cell Signaling Technology, 2678T, MA, United States), IL- 6 (1:1000, ABclonal, A0286, Wuhan, China), NFκB (1:5000, Abmart, T55034S, Shanghai, China), p- NF- κB (Ser536,1:1000, Cell Signaling Technology, 3033T, MA, United States), MyHC (1 μg/mL, R&D Systems, CAEI0822101, MN, United States), Atrogin1 (1:1000, Abways, CY8766, Shanghai, China) and MuRF1 (1:1000, Proteintech, 55 456- 1- AP, Wuhan, China), and then, an ECL plus kit was used to produce the blots (Proteintech, Wuhan, China). .. A chemiluminescence detection system (Syngene, Gene Gnome XRQ, Cambridge, Cambridgeshire, United Kingdom) was utilized to visualize the protein bands, and ImageJ software (NIH, MD, United States) was utilized for analysis.

    other:

    Article Title: Angelicin attenuates sepsis-associated splenic injury by targeting NF-κB/JAK2/STAT3 and PI3K/Akt pathways to inhibit inflammation and apoptosis.
    Article Snippet: Sepsis represents a clinical syndrome characterized by maladaptive host immune dysregulation in response to infection, leading to potentially fatal multiorgan dysfunction.. As the largest secondary lymphoid organ in mammals, spleen tissue plays a fundamental role in immune defense.. Angelicin (ANG), the main active ingredient in the traditional Chinese medicine Psoralea corylifolia Linn., possesses biological activities such as antiinflammation and anti-apoptosis.



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